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著作: [木戸 博]/Yokogoshi Yutaka/Sakai Kentaro/Tashiro Masato/Kishino Yasuo/Fukutomi Aiko/Katunuma Nobuhiko/Isolation and Characterization of A Novel Trypsin-like Protease Found in Rat Bronchiolar Epithelial Clara Cells: A Possible Activator of The Viral Fusion Glycoprotein/[The Journal of Biological Chemistry]

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EID
154814
EOID
936926
Map
0
LastModified
2018年12月19日(水) 10:52:23
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
木戸 博
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.分子酵素学研究センター(〜2007年3月31日/->組織[徳島大学.疾患酵素学研究センター])
著者 必須
  1. 木戸 博([徳島大学.先端酵素学研究所.重点研究部門])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Yokogoshi Yutaka
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Sakai Kentaro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Tashiro Masato
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Kishino Yasuo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Fukutomi Aiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Katunuma Nobuhiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Isolation and Characterization of A Novel Trypsin-like Protease Found in Rat Bronchiolar Epithelial Clara Cells: A Possible Activator of The Viral Fusion Glycoprotein

副題 任意
要約 任意

(英) A novel trypsin-like protease associated with rat bronchiolar epithelial Clara cells, named Tryptase Clara, was purified to homogeneity from rat lung by a series of standard chromatographic procedures. The enzyme has apparent molecular masses of 180 +/- 16 kDa on gel filtration and 30 +/- 1.5 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Its isoelectric point is pH 4.75. Studies with model peptide substrates showed that the enzyme preferentially recognizes a single arginine cleavage site, cleaving Boc-Gln-Ala-Arg-4-methylcoumaryl-7-amide most efficiently and having a pH optimum of 7.5 with this substrate. The enzyme is strongly inhibited by aprotinin, diisopropylfluorophosphate, antipain, leupeptin, and Kunitz-type soybean trypsin inhibitor, but inhibited only slightly by Bowman-Birk soybean trypsin inhibitor, benzamidine, and alpha 1-antitrypsin. Immunohistochemical studies indicated that the enzyme is located exclusively in the bronchiolar epithelial Clara cells and colocalized with surfactant. An immunoreactive protein with a molecular mass of 28.5 kDa was also detected in airway secretions by Western blotting analyses, suggesting that the 30-kDa protease in Clara cells is processed before or after its secretion. Proteolytic cleavage of the hemagglutinin of influenza virus is a prerequisite for the virus to become infectious. Tryptase Clara was shown to cleave the hemagglutinin and activate infectivity of influenza A virus in a dose-dependent way. These results suggest that the enzyme is a possible activator of inactive viral fusion glycoprotein in the respiratory tract and thus responsible for pneumopathogenicity of the virus.

キーワード 推奨
  1. (英) Amino Acid Sequence
  2. (英) Animals
  3. (英) Antibody Specificity
  4. (英) Blotting, Western
  5. (英) Bronchi
  6. (英) Cells, Cultured
  7. (英) Chromatography, Liquid
  8. (英) Electrophoresis, Polyacrylamide Gel
  9. (英) Epithelial Cells
  10. (英) Epithelium
  11. (英) Hydrolysis
  12. 免疫組織化学(immunohistochemistry)
  13. (英) Influenza A virus
  14. (英) Male
  15. (英) Molecular Sequence Data
  16. (英) Rats
  17. (英) Serine Endopeptidases
  18. (英) Substrate Specificity
  19. (英) Tryptases
  20. (英) Viral Fusion Proteins
発行所 推奨
誌名 必須 The Journal of Biological Chemistry([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
ISSN 任意 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 267
必須 19
必須 13573 13579
都市 任意
年月日 必須 1992年 4月 3日
URL 任意
DOI 任意
PMID 任意 1618859    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意 2-s2.0-0026689352
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article

  2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't