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著作: Shinohara Hisashi/[矢野 聖二]/Bucana D. Corazon/Fidler J. Isaiah/Induction of Chemokine Secretion and Enhancement of Contact-Dependent Macrophage Cytotoxicity by Engineered Expression of Granulocyte-Macrophage Colony-Stimulating Factor in Human Colon Cancer Cells/[The Journal of Immunology]

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EID
146323
EOID
933831
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LastModified
2018年12月3日(月) 13:13:37
Operator
大家 隆弘
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[研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. (英) Shinohara Hisashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 矢野 聖二
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Bucana D. Corazon
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Fidler J. Isaiah
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Induction of Chemokine Secretion and Enhancement of Contact-Dependent Macrophage Cytotoxicity by Engineered Expression of Granulocyte-Macrophage Colony-Stimulating Factor in Human Colon Cancer Cells

副題 任意
要約 任意

(英) We investigated the role of tumor cell-derived GM-CSF in recruitment and tumoricidal activation of tissue macrophages. Transfection of the murine GM-CSF gene into KM12SM human colon cancer cells decreased the tumorigenicity of transfected cells and nontransfected bystander colon cancer cells in nude mice. Sequential tissue sections from sites injected with high GM-CSF-producing tumor cells (but not from nontransfected or low GM-CSF-producing cells) demonstrated a dense infiltration of polymorphonuclear cells (PMN), followed by infiltration of macrophages, which correlated with expression of the macrophage-inflammatory protein-1alpha and the monocyte chemoattractant protein-1 (MCP-1) in mouse PMN and macrophages. GM-CSF-producing KM12SM cells were highly sensitive to lysis by mouse macrophages and also increased macrophage-mediated lysis of bystander nontransfected KM12SM cells. The incubation of macrophages with GM-CSF induced expression of the CD11b surface adhesion molecule, which was associated with increased attachment to tumor cells. All KM12SM cells were sensitive to macrophage-mediated lysis in the presence of rGM-CSF and recombinant MCP-1. Collectively, the results demonstrate that tumor cell-derived GM-CSF stimulates PMN and macrophages to secrete macrophage-inflammatory protein-1alpha and MCP-1, which triggers recruitment of mononuclear cells, induces expression of adhesion molecules on macrophages, and enhances contact-dependent cytolysis of tumor cells.

キーワード 推奨
  1. (英) Animals
  2. (英) Cell Communication
  3. (英) Cell Movement
  4. (英) Chemokine CCL2
  5. (英) Chemokine CCL4
  6. (英) Chemokines
  7. (英) Coculture Techniques
  8. (英) Colonic Neoplasms
  9. (英) Cytotoxicity, Immunologic
  10. (英) Gene Expression Regulation, Neoplastic
  11. (英) Granulocyte-Macrophage Colony-Stimulating Factor
  12. (英) HT29 Cells
  13. (英) Humans
  14. (英) Injections, Subcutaneous
  15. (英) Macrophage Inflammatory Proteins
  16. (英) Macrophage-1 Antigen
  17. (英) Macrophages
  18. (英) Male
  19. (英) Mice
  20. (英) Mice, Nude
  21. (英) Neoplasm Transplantation
  22. (英) Neutrophils
  23. (英) Protein Engineering
  24. (英) Recombinant Proteins
  25. (英) Transfection
  26. (英) Tumor Cells, Cultured
発行所 推奨
誌名 必須 The Journal of Immunology([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)
ISSN 任意 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 164
必須 5
必須 2728 2737
都市 任意
年月日 必須 2000年 3月 1日
URL 任意 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10679114&dopt=Abstract
DOI 任意 10.4049/jimmunol.164.5.2728    (→Scopusで検索)
PMID 任意 10679114    (→Scopusで検索)
NAID 任意
WOS 任意 000085477100056
Scopus 任意 2-s2.0-0034163565
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article

  2. (英) Article.PublicationTypeList.PublicationType: Research Support, U.S. Gov't, P.H.S.