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著作: 村上 政隆/Murdiastuti, Kwartarini/[細井 和雄]/Hill, E Adrian/AQP and the control of fluid transport in a salivary gland/[The Journal of Membrane Biology]

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EID
145145
EOID
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LastModified
2017年11月29日(水) 17:45:21
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大家 隆弘
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細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
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  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
著者 必須
  1. (英) Murakami, Masataka / (日) 村上 政隆 / (読) むらかみ まさたか
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Murdiastuti, Kwartarini
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Hill, E Adrian
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) AQP and the control of fluid transport in a salivary gland

副題 任意
要約 任意

(英) Experiments were performed with the perfused rat submandibular gland in vitro to investigate the nature of the coupling between transported salt and water by varying the osmolarity of the source bath and observing the changes in secretory volume flow. Glands were submitted to hypertonic step changes by changing the saline perfusate to one containing different levels of sucrose. The flow rate responded by falling to a lower value, establishing a new steady-state flow. The rate changes did not correspond to those expected from a system in which fluid production is due to simple osmotic equilibration, but were much larger. The changes were fitted to a model in which fluid production is largely paracellular, the rate of which is controlled by an osmosensor system in the basal membrane. The same experiments were done with glands from rats that had been bred to have very low levels of AQP5 (the principal aquaporin of the salivary acinar cell) in which little AQP5 is expressed at the basal membrane. In these rats, salivary secretion rates after hypertonic challenges were small and best modelled by simple osmotic equilibration. In rats which had intermediate AQP5 levels the changes in flow rate were similar to those of normal rats although their AQP5 levels were reduced.Finally, perfused normal glands were subject to retrograde ductal injection of salines containing different levels of Hg(2+) ions (0, 10 and 100 microM: ) which would act as inhibitors of AQP5 at the apical acinar membrane. The overall flow rates were progressively diminished with rising Hg(2+) concentration, but after hypertonic challenge the changes in flow rates were unchanged and similar to those of normal rats. All these results are difficult to explain by a cellular osmotic model but can be explained by a model in which paracellular flow is controlled by an osmosensor (presumably AQP5) present on the basal membrane.

キーワード 推奨
  1. (英) Animals
  2. (英) Aquaporin 5
  3. (英) Biological Transport
  4. (英) Cell Membrane Permeability
  5. (英) Mercury
  6. (英) Models, Biological
  7. (英) Organ Culture Techniques
  8. (英) Osmolar Concentration
  9. (英) Perfusion
  10. (英) Rats
  11. (英) Rats, Sprague-Dawley
  12. (英) Salivation
  13. (英) Submandibular Gland
発行所 推奨 (英) Springer New York
誌名 必須 The Journal of Membrane Biology([Springer-Verlag])
(pISSN: 0022-2631, eISSN: 1432-1424)
ISSN 任意 0022-2631
ISSN: 0022-2631 (pISSN: 0022-2631, eISSN: 1432-1424)
Title: The Journal of membrane biology
Title(ISO): J Membr Biol
Supplier: Springer Online Journal Archive
Publisher: Springer New York
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 210
必須 2
必須 91 103
都市 任意
年月日 必須 2006年 3月 初日
URL 任意
DOI 任意 10.1007/s00232-005-0848-2    (→Scopusで検索)
PMID 任意 16868676    (→Scopusで検索)
NAID 任意
WOS 任意 000239818500003
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Center for Integrative Bioscience, NIPS, National Institute for Natural Sciences, Myoudaiji, Okazaki 444-8787, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article