徳島大学 教育・研究者情報データベース(EDB)

1. (英) Chowdhury Mohammed Golam Mowla

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2. 前田 健一

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3. 安友 康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])

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4. 前川 洋一

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5. (英) Furukawa Atsuko

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6. (英) Azuma Miyuki

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7. (英) Nagasawa Hideyuki

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8. (英) Himeno Kunisuke

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(英) Antigen-specific B cells are required for the secondary response of T cells but not for their priming

(英) We studied the potential role of B cells in T cell responses using severe-combined immunodeficient (SCID) mice grafted with the thymus from fetal C.B-17 mice (TG mice). These mice developed both CD4+ and CD8+ T cells, but not B cells within 2 months after transplantation. TG mice showed normal delayed-type hypersensitivity responses against the immunizing antigen ovalbumin (OVA). Lymph node (LN) cells of TG mice proliferated well in response to concanavalin A (Con A). Further, Con A stimulation induced the production of interleukin (IL)-2, IL-6 and interferon (IFN)-gamma and the expression of IL-4 mRNA. Thus, TG mice were reconstituted without remarkable immunodeficiency. However, these T cells failed to proliferate to OVA stimulation. Response to OVA was also inhibited in SCID mice grafted with fetal C.B-17 liver cells when B cells were depleted in the proliferation assay. Unresponsiveness against immunizing antigen was restored by the addition of antigen-primed B cells, but not by naive B cells, lipopolysaccharide-activated B cells or B cells primed with sheep red blood cells. Next, we examined whether antigen-primed B cells could induce T cell responses without professional antigen-presenting cells (APC). T and B cells were purified from OVA-immunized mice by cell sorter. These T cells proliferated in response to OVA and produced IFN-gamma in the absence of non-B APC. When anti-CD80 or anti-CD86 was added in the assay, proliferation and IFN-gamma production was inhibited. These results indicate that B cells activated specifically with antigen are required for the secondary response of T cells, but not for their priming.

1. (英) Animals
2. (英) Antigen-Presenting Cells
3. (英) Antigens, CD28
4. (英) Antigens, CD80
5. (英) B-Lymphocytes
6. (英) Epitopes
7. (英) Fetal Tissue Transplantation
8. 造血(hematopoiesis)
9. (英) Hypersensitivity, Delayed
10. (英) Immunization, Secondary
11. (英) Liver Transplantation
12. (英) Lymphocyte Activation
13. (英) Mice
14. (英) Mice, SCID
15. (英) Ovalbumin
16. Tリンパ球(T lymphocytes)
17. (英) Thymus Gland

1. (英) Article.PublicationTypeList.PublicationType: Journal Article