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著作: [柴田 洋文]/Odahara Y/Sasaki F/Hirota O/Nishida M/Tani I/The presence of low molecular weight germinative substances in Bacillus cereus T spore extract/[Microbiology and Immunology]

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EID
139154
EOID
784102
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LastModified
2015年7月16日(木) 11:40:33
Operator
三木 ちひろ
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[副研究部長]/[徳島大学.大学院ヘルスバイオサイエンス研究部]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
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学究種別 推奨
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著者 必須
  1. 柴田 洋文
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Odahara Y
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Sasaki F
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Hirota O
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Nishida M
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Tani I
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) The presence of low molecular weight germinative substances in Bacillus cereus T spore extract

副題 任意
要約 任意

(英) An extract from intact spores of Bacillus cereus T having a germination-inducing activity was studied. Two distinct germinative principles were found through dialysis of the extract. One was diffusible through the dialysis membrane and the other was non-diffusible. The activity of the former fraction was inhibited by the addition of 1 mM glycoletherdiamine-N,N,N',N'-tetraacetic acid (GEDTA), whereas the latter fraction was inactive unless GEDTA was added to the assay system. The diffusible principle maintained the major portion of the activity found in the crude spore extract. By means of high-performance liquid chromatography (HPLC) using a gel permeation chromatography column, 9 fractions were obtained from the deproteinized diffusible fraction. Of those fractions, two fractions (No. 1 and No. 8) were responsible for the germination-inducing activity, but no reconstituted activity was observed unless both fractions No. 1 and No. 8 were added to the assay system. Amino acid analysis of fraction No. 1 revealed that the fraction was rich in free amino acids, especially in alanine. On the other hand, by the use of reverse-phase HPLC and fast atom bombardment mass spectrometry, it was concluded that the effective substance in fraction No. 8 was inosine. Based on these findings, it was suggested that the active substances in fraction No. 1 might be a free amino acid such as L-alanine and/or Ca2+ and a Ca(2+)-binding substance.

キーワード 推奨
  1. (英) Alanine
  2. (英) Amino Acids
  3. (英) Bacillus cereus
  4. (英) Chromatography, High Pressure Liquid
  5. 拡散(diffusion)
  6. (英) Egtazic Acid
  7. (英) Inosine
  8. 分子量(molecular weight)
  9. (英) Spectrometry, Mass, Fast Atom Bombardment
  10. (英) Spores, Bacterial
発行所 推奨 日本細菌学会
誌名 必須 Microbiology and Immunology([日本細菌学会])
(pISSN: 0385-5600, eISSN: 1348-0421)
ISSN 任意 0385-5600
ISSN: 0385-5600 (pISSN: 0385-5600, eISSN: 1348-0421)
Title: Microbiology and immunology
Title(ISO): Microbiol Immunol
Supplier: 財団法人 学会誌刊行センター
Publisher: Wiley Publishing
 (NLM Catalog  (医中誌Web  (J-STAGE  (Wiley  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 38
必須 7
必須 519 525
都市 任意
年月日 必須 1994年 0月 初日
URL 任意
DOI 任意
PMID 任意 7968684    (→Scopusで検索)
CRID 任意
WOS 任意
Scopus 任意 2-s2.0-0028040688
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指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article