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著作: [新垣 尚捷]/Nagao Tomoko/NIki Rie/Toyofuku Ayako/Tanaka Hiroaki/Kuramoto Yoshinori/Emoto Yuka/[柴田 洋文]/Magota Koji/[樋口 富彦]/Possible role of cell surface H+-ATP synthase in the extracellular ATP synthesis and proliferation of human umbilical vein endothelial cells/[Molecular Cancer Research : MCR]

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EID
139135
EOID
784165
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0
LastModified
2015年7月16日(木) 16:27:27
Operator
三木 ちひろ
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TRUE
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Owner
新垣 尚捷
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 新垣 尚捷
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Nagao Tomoko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) NIki Rie
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Toyofuku Ayako
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Tanaka Hiroaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Kuramoto Yoshinori
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Emoto Yuka
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. 柴田 洋文
    役割 任意
    貢献度 任意
    学籍番号 推奨
  9. (英) Magota Koji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  10. 樋口 富彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Possible role of cell surface H+-ATP synthase in the extracellular ATP synthesis and proliferation of human umbilical vein endothelial cells

副題 任意
要約 任意

(英) Extracellular ATP synthesis on human umbilical vein endothelial cells (HUVECs) was examined, and it was found that HUVECs possess high ATP synthesis activity on the cell surface. Extracellular ATP generation was detected within 5 s after addition of ADP and inorganic phosphate and reached a maximal level at 15 s. This type of ATP synthesis was almost completely inhibited by mitochondrial H(+)-ATP synthase inhibitors (e.g., efrapeptins, resveratrol, and piceatannol), which target the F(1) catalytic domain. Oligomycin and carbonyl cyanide m-chlorophenylhydrazone, but not potassium cyanide, also inhibited extracellular ATP synthesis on HUVECs, suggesting that cell surface ATP synthase employs the transmembrane electrochemical potential difference of protons to synthesize ATP as well as mitochondrial H(+)-ATP synthase. The F(1)-targeting H(+)-ATP synthase inhibitors markedly inhibited the proliferation of HUVECs, but intracellular ATP levels in HUVECs treated with these inhibitors were only slightly affected, as shown by comparison with the control cells. Interestingly, piceatannol inhibited only partially the activation of Syk (a nonreceptor tyrosine kinase), which has been shown to play a role in a number of endothelial cell functions, including cell growth and migration. These findings suggest that H(+)-ATP synthase-like molecules on the surface of HUVECs play an important role not only in extracellular ATP synthesis but also in the proliferation of HUVECs. The present results demonstrate that the use of small molecular H(+)-ATP synthase inhibitors targeting the F(1) catalytic domain may lead to significant advances in potential antiangiogenic cancer therapies.

キーワード 推奨
  1. (英) Adenosine Triphosphate
  2. (英) Carbonyl Cyanide m-Chlorophenyl Hydrazone
  3. (英) Cell Division
  4. (英) Cell Line
  5. (英) Cell Membrane
  6. (英) Dose-Response Relationship, Drug
  7. (英) Endothelial Cells
  8. (英) Enzyme Precursors
  9. (英) Extracellular Space
  10. (英) Humans
  11. (英) Intracellular Signaling Peptides and Proteins
  12. (英) Oligomycins
  13. (英) Peptides
  14. (英) Potassium Cyanide
  15. (英) Protein-Tyrosine Kinases
  16. (英) Proton-Translocating ATPases
  17. (英) Ribonucleotide Reductases
  18. (英) Stilbenes
  19. (英) Umbilical Veins
発行所 推奨 American Association for Cancer Research
誌名 必須 Molecular Cancer Research : MCR([American Association for Cancer Research])
(pISSN: 1541-7786, eISSN: 1557-3125)
ISSN 任意 1541-7786
ISSN: 1541-7786 (pISSN: 1541-7786, eISSN: 1557-3125)
Title: Molecular cancer research : MCR
Title(ISO): Mol Cancer Res
Publisher: American Association for Cancer Research
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 1
必須 13
必須 931 939
都市 任意
年月日 必須 2003年 11月 初日
URL 任意 http://mcr.aacrjournals.org/cgi/content/abstract/1/13/931
DOI 任意
PMID 任意 14638865    (→Scopusで検索)
NAID 任意
WOS 任意 000186789000001
Scopus 任意 2-s2.0-0344011492
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Faculty of Pharmaceutical Sciences, University of Tokushima, Shomachi, Tokushima, Japan. arakaki@ph.tokushima-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't