徳島大学 教育・研究者情報データベース(EDB)

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著作: Masunaga Shin-ichiro/[宇都 義浩]/[永澤 秀子]/[堀 均]/Nagata Kenji/Suzuki Minoru/Kinashi Yuko/Ono Koji/Evaluation of Hypoxic Cell Radio-sensitizers in Terms of Radio-sensitizing and Repair-inhibiting Potential Dependency on p53 Status of Tumor Cells and the Effects on Intratumor Quiescent Cells/[Anticancer Research]

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EID
136820
EOID
669595
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2012年11月6日(火) 14:11:41
Operator
大家 隆弘
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宇都 義浩
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.工学部.生物工学科.生物機能工学講座
著者 必須
  1. (英) Masunaga Shin-ichiro
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 宇都 義浩([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.応用生物資源学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 永澤 秀子(岐阜薬科大学/->個人[紺世 秀子])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 堀 均
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Nagata Kenji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Suzuki Minoru
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Kinashi Yuko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. (英) Ono Koji
    データベース中に適合する可能性のある以下の情報を発見しました
    [個人] 小野 公嗣
    この情報が上記に掲げた個人の業績等に分類されるためには参照登録が必要です.上記に掲げた個人本人に該当する場合には参照登録に変更してください.
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Evaluation of Hypoxic Cell Radio-sensitizers in Terms of Radio-sensitizing and Repair-inhibiting Potential Dependency on p53 Status of Tumor Cells and the Effects on Intratumor Quiescent Cells

副題 任意
要約 任意

(英) Intratumor quiescent (Q) cells and p53-mutated tumor cells are more difficult to control than intratumor proliferating (P) cells and p53 wild-type tumor cells, respectively. The usefulness of 3 hypoxic cell radio-sensitizers was compared in terms of a radio-sensitizing effect under aerobic and hypoxic conditions and a repair-inhibiting effect following irradiation on both Q and total (P + Q) cell populations in solid tumors. The dependency of these effects on the p53 status of tumor cells was also examined using tumor cell lines with identical genetic backgrounds except for their p53 status. Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mp53) or with neo vector as a control (SAS/neo) were inoculated subcutaneously into both the hind legs of Balb/cA nude mice. The nude mice bearing the tumors and C3H/He mice bearing SCC VII tumors received 5-bromo-2'-deoxyuridine (BrdU) continuously to label all the P cells in the tumors. Tumor-bearing mice received gamma-ray irradiation while alive or following tumor clamping after being administered no drug, nimorazole, SR-2514 or misonidazole, or received no drug, nimorazole, SR-2514 or misonidazole straight after gamma-ray irradiation. For the group irradiated after receiving the drug, the tumors were excised immediately following irradiation, while for the group irradiated before receiving the drug, the tumors were excised 24 h after irradiation. The excised tumors were minced and trypsinized. The tumor cell suspensions thus obtained were incubated with cytochalasin-B (a cytokinesis blocker), and the micronucleus (MN) frequency in the cells without BrdU labelling (= quiescent (Q) cells) was determined using immunofluorescence staining for BrdU. Meanwhile, the MN frequency in the total tumor cell population was determined from the tumors that had not been pretreated with BrdU. The clonogenic cell survival was also determined in the mice given no BrdU. Both the radio-sensitizing effects under aerobic and hypoxic conditions and the repair-inhibiting effects following gamma-ray irradiation increased in the following order: nimorazole < SR-2514 < misonidazole in both total and Q cells in these 3 tumors. Both effects were more marked in the Q cells and p53-mutated tumors than in the total cells and p53-wild tumors, respectively. In terms of controlling radio-resistant Q tumor cells and p53-mutated tumor cells, the combination of radio-sensitizers and conventional radiotherapy is promising both for radio-sensitization and for repair-inhibition, but further study of the toxicity to normal tissues is needed.

キーワード 推奨
  1. (英) Animals
  2. (英) Antineoplastic Agents
  3. (英) Carcinoma, Squamous Cell
  4. (英) Cell Hypoxia
  5. (英) Combined Modality Therapy
  6. (英) DNA Repair
  7. (英) Dose-Response Relationship, Radiation
  8. (英) Female
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] 女性 (female)
  9. (英) Gamma Rays
  10. (英) Genes, p53
  11. (英) Head and Neck Neoplasms
  12. (英) Humans
  13. (英) Mice
    データベース中に適合する可能性のある以下の情報を発見しました
    [キーワード] ノックアウトマウス (knockout mice)
  14. (英) Mice, Inbred BALB C
  15. (英) Mice, Inbred C3H
  16. (英) Misonidazole
  17. (英) Nimorazole
  18. (英) Radiation-Sensitizing Agents
  19. (英) Xenograft Model Antitumor Assays
発行所 推奨
誌名 必須 Anticancer Research(International Institute of Anticancer Research(IIAR))
(pISSN: 0250-7005, eISSN: 1791-7530)
ISSN 任意 0250-7005
ISSN: 0250-7005 (pISSN: 0250-7005, eISSN: 1791-7530)
Title: Anticancer research
Title(ISO): Anticancer Res.
Publisher: International Institute of Anticancer Research
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 26
必須 2A
必須 1261 1270
都市 任意
年月日 必須 2006年 3月 初日
URL 任意
DOI 任意
PMID 任意 16619533    (→Scopusで検索)
NAID 任意
WOS 任意 000236674800060
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Radiation Oncology Research Laboratory, Research Reactor Institute, Kyoto University, 2-1010, Asashiro-nishi, Kumatori-cho, Sennan-gun, Osaka 590-0494, Japan. smasuna@rri.kyoto-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: Evaluation Studies

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't

  5. (英) MedlineDate: 2006 Mar-Apr