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著作: [前川 正彦]/Kamada Masaharu/[山本 哲史]/[山野 修司]/[苛原 稔]/[木戸 博]/[青野 敏博]/Involvement of carbohydrate molecules on zona pellucida in human fertilization/[Journal of Reproductive Immunology]

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EID
135860
EOID
701164
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LastModified
2013年8月12日(月) 14:24:15
Operator
大家 隆弘
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苛原 稔
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
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組織 推奨
著者 必須
  1. 前川 正彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Kamada Masaharu
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 山本 哲史
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 山野 修司
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 苛原 稔
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 木戸 博([徳島大学.先端酵素学研究所.重点研究部門])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 青野 敏博
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Involvement of carbohydrate molecules on zona pellucida in human fertilization

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要約 任意

(英) Although the involvement of several receptors and ligand molecules in sperm-zona interaction in many species have been proposed, there has been a little analysis of the kinetics between these molecules during the interaction. In the present study, we applied the detection method using surface plasmon resonance (SPR) by a BIAcore apparatus for the analysis of the putative receptor-ligand interaction of sperm-egg binding. Mannose-BSA or [man](5)-[GlcNAc](2)-Asp was immobilized on the surface of a sensor chip. When concanavalin A (Con A) was delivered to each of two different sensor chips to evaluate their usefulness, the resonance signal after sample injection onto a [man](5)-[GlcNAc](2)-Asp-fixed chip decreased rapidly than the mannose-BSA-fixed chip. However, the amount of binding for Con A during the injection onto the [man](5)-[GlcNAc](2)-Asp-fixed chip was high. When acid sperm extracts (acid extracts) and fractions through a CM column, containing protease activity (protease fractions), were delivered to the mannose-BSA-fixed chip, the SPR signal during the injection was not obviously changed compared with that of the control. However, when sperm samples were delivered to the [man](5)-[GlcNAc](2)-Asp-fixed chip, the SPR response during the injection was enormous. These results suggest that the [man](5)-[GlcNAc](2)-Asp-fixed chip is more useful than the mannose-BSA-fixed chip for investigating the interactions with sperm extracts and that the sensitive method using SPR by a BIAcore apparatus is applicable for the analysis of the putative receptor-ligand interaction of sperm-egg binding.

キーワード 推奨
  1. (英) Mannose
  2. (英) Surface plasmon resonance
  3. (英) Sperm-Zona interaction
  4. (英) Lectin-carbohydrate binding
発行所 推奨 Elsevier(->組織[Elsevier Science])
誌名 必須 Journal of Reproductive Immunology(European Society of Reproductive Immunology)
(pISSN: 0165-0378, eISSN: 1872-7603)
ISSN 任意 0165-0378
ISSN: 0165-0378 (pISSN: 0165-0378, eISSN: 1872-7603)
Title: Journal of reproductive immunology
Title(ISO): J Reprod Immunol
Publisher: Elsevier Ireland Ltd
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 53
必須 1-2
必須 79 89
都市 任意
年月日 必須 2002年 1月 初日
URL 任意
DOI 任意 10.1016/S0165-0378(01)00096-1    (→Scopusで検索)
PMID 任意 11730906    (→Scopusで検索)
CRID 任意
WOS 任意 000172873300008
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, 3-18-15 Kuramoto-cho, Tokushima 770-8503, Japan. masahiko@clin.med.tokushima-u.ac.jp

  2. (英) Article.PublicationTypeList.PublicationType: In Vitro

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't