著作: [前川 正彦]/Kamada Masaharu/[山本 哲史]/[山野 修司]/[苛原 稔]/[木戸 博]/[青野 敏博]/Involvement of carbohydrate molecules on zona pellucida in human fertilization/[Journal of Reproductive Immunology]
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種別 | 必須 | 学術論文(審査論文) | |||
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言語 | 必須 | 英語 | |||
招待 | 推奨 | ||||
審査 | 推奨 | ||||
カテゴリ | 推奨 | ||||
共著種別 | 推奨 | ||||
学究種別 | 推奨 | ||||
組織 | 推奨 | ||||
著者 | 必須 | ||||
題名 | 必須 |
(英) Involvement of carbohydrate molecules on zona pellucida in human fertilization |
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副題 | 任意 | ||||
要約 | 任意 |
(英) Although the involvement of several receptors and ligand molecules in sperm-zona interaction in many species have been proposed, there has been a little analysis of the kinetics between these molecules during the interaction. In the present study, we applied the detection method using surface plasmon resonance (SPR) by a BIAcore apparatus for the analysis of the putative receptor-ligand interaction of sperm-egg binding. Mannose-BSA or [man](5)-[GlcNAc](2)-Asp was immobilized on the surface of a sensor chip. When concanavalin A (Con A) was delivered to each of two different sensor chips to evaluate their usefulness, the resonance signal after sample injection onto a [man](5)-[GlcNAc](2)-Asp-fixed chip decreased rapidly than the mannose-BSA-fixed chip. However, the amount of binding for Con A during the injection onto the [man](5)-[GlcNAc](2)-Asp-fixed chip was high. When acid sperm extracts (acid extracts) and fractions through a CM column, containing protease activity (protease fractions), were delivered to the mannose-BSA-fixed chip, the SPR signal during the injection was not obviously changed compared with that of the control. However, when sperm samples were delivered to the [man](5)-[GlcNAc](2)-Asp-fixed chip, the SPR response during the injection was enormous. These results suggest that the [man](5)-[GlcNAc](2)-Asp-fixed chip is more useful than the mannose-BSA-fixed chip for investigating the interactions with sperm extracts and that the sensitive method using SPR by a BIAcore apparatus is applicable for the analysis of the putative receptor-ligand interaction of sperm-egg binding. |
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キーワード | 推奨 |
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発行所 | 推奨 | Elsevier(->組織[Elsevier Science]) | |||
誌名 | 必須 |
Journal of Reproductive Immunology(European Society of Reproductive Immunology)
(pISSN: 0165-0378, eISSN: 1872-7603)
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巻 | 必須 | 53 | |||
号 | 必須 | 1-2 | |||
頁 | 必須 | 79 89 | |||
都市 | 任意 | ||||
年月日 | 必須 | 2002年 1月 初日 | |||
URL | 任意 | ||||
DOI | 任意 | 10.1016/S0165-0378(01)00096-1 (→Scopusで検索) | |||
PMID | 任意 | 11730906 (→Scopusで検索) | |||
CRID | 任意 | ||||
WOS | 000172873300008 | ||||
Scopus | 任意 | ||||
researchmap | 任意 | ||||
評価値 | 任意 | ||||
被引用数 | 任意 | ||||
指導教員 | 推奨 | ||||
備考 | 任意 |
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