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著作: Ohksa Tomoko/[野間 隆文]/Ueyama Takeshi/Hisamatsu Yuji/Yano Masafumi/Esato Kensuke/Nakazawa Atsushi/Matsuzaki Masunori/Differences in sarcoplasmic reticulum gene expression in myocardium from patients undergoing cardiac surgery. Quantification of steady-state levels of messenger RNA using the reverse transcription-polymerase chain reaction/[Heart and Vessels]

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2014年2月24日(月) 18:18:30
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大家 隆弘
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野間 隆文
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種別 必須 学術論文(審査論文)
言語 必須 英語
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著者 必須
  1. (英) Ohksa Tomoko
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  2. 野間 隆文([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.分子医化学])
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  3. (英) Ueyama Takeshi
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  4. (英) Hisamatsu Yuji
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  5. (英) Yano Masafumi
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  6. (英) Esato Kensuke
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  7. (英) Nakazawa Atsushi
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  8. (英) Matsuzaki Masunori
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題名 必須

(英) Differences in sarcoplasmic reticulum gene expression in myocardium from patients undergoing cardiac surgery. Quantification of steady-state levels of messenger RNA using the reverse transcription-polymerase chain reaction

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(英) Little is known about any alterations in sarcoplasmic reticulum (SR) gene expression associated with cardiac diseases of varying degrees of severity. We assessed, using the reverse transcription-polymerase chain reaction (RT-PCR) technique, SR Ca2+ transport protein gene expression in small tissue samples from failing hearts in patients undergoing cardiac surgery. Total RNA was extracted from 30- to 50-mg samples from the hearts of 13 patients with coronary artery disease, congenital heart disease, or valvular heart disease. We used RT-PCR to synthesize and amplify cDNA encoding cardiac SR Ca(2+)-ATPase, ryanodine receptor (RYR), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The amount of each mRNA in the sample was expressed relative to the amount of GAPDH mRNA. The expression level of each mRNA was correlated with the cardiac functional index. The mRNA levels for Ca(2+)-ATPase and RYR varied between heart samples, but showed a positive correlation with left ventricular ejection fraction. Ca(2+)-ATPase mRNA levels showed in inverse relationship with plasma brain natriuretic peptide. In addition, we isolated partial cDNA encoding a human cardiac RYR. The cDNA consisted of 487 nucleotides, and the nucleotide and deduced amino acid sequences showed 93% and 99% homology, respectively, to those of rabbit cardiac RYR. These results suggest that decreased levels of mRNA for SR Ca2+ transport protein could be related to abnormal cardiac function, regardless of the etiology of the heart disease. RT-PCR provides a rapid and economical way of quantifying the expression of multiple genes in small specimens and may, therefore, aid understanding of the pathophysiology and treatment of heart disease.

キーワード 推奨
  1. (英) human heart failure
  2. (英) sarcoplasmic reticulum
  3. (英) ryanodine receptor
  4. (英) Ca(2+)-ATPase
  5. (英) RT-PCR
発行所 推奨
誌名 必須 Heart and Vessels(Japanese Research Promotion Society for Cardiovascular Diseases)
(pISSN: 0910-8327, eISSN: 1615-2573)
ISSN 任意 0910-8327
ISSN: 0910-8327 (pISSN: 0910-8327, eISSN: 1615-2573)
Title: Heart and vessels
Title(ISO): Heart Vessels
Supplier: Springer Online Journal Archive
Publisher: Springer Japan
 (NLM Catalog  (医中誌Web  (Scopus  (CrossRef (Scopus information is found. [need login])
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年月日 必須 1997年 0月 初日
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PMID 任意 9288554    (→Scopusで検索)
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  1. (英) Article.DataBankList.DataBank.DataBankName: GENBANK

  2. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: D83212

  3. (英) Article.PublicationTypeList.PublicationType: Journal Article

  4. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't