著作: Asai Takashi/Ohkubo Takayoshi/Katsuya Tomohiro/Higaki Jitsuo/Fu Yuxiao/Fukuda Masayuki/Hozawa Atsushi/Matsubara Mitsunobu/Kitaoka Hirofumi/Tsuji Ichiro/[荒木 勉]/Satoh Hiroshi/Hisamichi Shigeru/Imai Yutaka/Ogihara Toshio/Endothelin-1 Gene Variant Associates With Blood Pressure in Obese Japanese Subjects: The Ohasama Study/[Hypertension]
(英) Endothelin-1 Gene Variant Associates With Blood Pressure in Obese Japanese Subjects: The Ohasama Study
(英) A recent report based on the results of 2 epidemiological studies, the Etude Cas-Temoin de l'Infarctus Myocarde (ECTIM) and the Glasgow Heart Scan Study, revealed that a G/T polymorphism with an amino acid substitution (Lys-->Asn) at codon 198 in exon 5 of the endothelin-1 gene (ET-1) is associated with blood pressure in overweight people. They suggested that G/T polymorphism of ET-1 strongly interacted with body mass index (BMI) in the determination of BP levels. To examine interaction among G/T polymorphism of ET-1, BMI, and BP, we performed an association study in a general Japanese population. Subjects (n=1250) were recruited from Ohasama, a cohort in a rural community of northern Japan. DNA was extracted from buffy coat of participants, and G/T polymorphism of ET-1 was determined by the TaqMan probe polymerase chain reaction method, a powerful tool for semiautomatic genotype determination of a large number of samples. Frequency of T (Asn 198) allele in Japanese (27%) was slightly but significantly higher than in whites (24%). Baseline characteristics (age, BMI, systolic and diastolic BP, and antihypertensive treatment) of all subjects were not significantly different according to the genotype of G/T polymorphism. However, in obese subjects (> or =25 kg/m(2)) diastolic BPs were significantly associated with G/T polymorphism of ET-1. After adjustment for confounding factors, significant association remained; for overweight subjects, diastolic BP level in those with T allele (GT + TT) was 1.8 mm Hg (P=0.04) higher than in those with GG genotype. That similar results were obtained from subjects of different races suggests that the Lys198Asn polymorphism of ET-1 is involved in determination of BP levels in obese subjects.
|発行所||推奨||American Heart Association|
Hypertension([American Heart Association])
|年月日||必須||2001年 12月 1日|