徳島大学 教育・研究者情報データベース(EDB)

1. (英) Suryono

   役割	任意
   貢献度	任意
   学籍番号	推奨

2. 木戸 淳一

   役割	任意
   貢献度	任意
   学籍番号	推奨

3. (英) Hayashi Noriko

   役割	任意
   貢献度	任意
   学籍番号	推奨

4. 片岡 正俊

   役割	任意
   貢献度	任意
   学籍番号	推奨

5. 篠原 康雄([徳島大学.先端酵素学研究所.基幹研究部門])

   役割	任意
   貢献度	任意
   学籍番号	推奨

6. 永田 俊彦

   役割	任意
   貢献度	任意
   学籍番号	推奨

(英) Norepinephrine Stimulates Calprotectin Expression in Human Monocytic Cells

(英) Calprotectin is composed of two proteins, S100A8 and S100A9, which are S100 family members, and is detected in gingival crevicular fluid and gingival tissue with inflammation. The release and production of calprotectin are regulated by lipopolysaccharides of periodontopathic bacteria and cytokines. Emotional or psychological stress, a risk factor of periodontal disease, is transmitted by stress modulators including norepinephrine and cortisol. The aim of the present study was to investigate the effect of stress on calprotectin expression using norepinephrine and cortisol. U-937 cells, a human monocytic cell line, were incubated with norepinephrine in the presence or absence of beta- or alpha-adrenergic receptor antagonists, or with cortisol. The expression of S100A8/S100A9 mRNAs was examined by northern blotting and the amount of calprotectin was measured by enzyme-linked immunosorbent assay (ELISA). The DNA binding activity of C/EBPalpha (CCAAT enhancing binding protein), a transcription factor, was examined by electrophoretic mobility shift assay. Norepinephrine stimulated the expression of S100A8/S100A9 mRNAs via beta-adrenergic receptors in U-937 cells and significantly increased calprotectin production to about 3.6-fold that of the control. However, cortisol had no effect on calprotectin expression at the mRNA and protein levels. Norepinephrine elevated C/EBPalpha DNA binding activity, but cortisol did not increase the activity. Norepinephrine, a stress modulator, stimulated calprotectin expression in human monocytic cells. Calprotectin expression may be regulated by stress in addition to inflammatory factors.

1. (英) Calprotectin
2. (英) Norepinephrine
3. (英) Cortisol
4. ストレス(stress)

1. (英) Article.Affiliation: Department of Periodontology, Faculty of Dentistry, Gadjah Mada University, Yogyakarta, Indonesia.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't