著作: [片岡 正俊]/Shimizu Yasuki/Kunikiyo Kenji/Asahara Yoji/Azuma Hideki/Sawa Takamasa/[木戸 淳一]/[永田 俊彦]/Nifedipine induces gingival overgrowth in rats through a reduction in collagen phagocytosis by gingival fibroblasts/[Journal of Periodontology]
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削除する| 種別 | 必須 | 学術論文(審査論文) | |||
|---|---|---|---|---|---|
| 言語 | 必須 | 英語 | |||
| 招待 | 推奨 | ||||
| 審査 | 推奨 | ||||
| カテゴリ | 推奨 | ||||
| 共著種別 | 推奨 | ||||
| 学究種別 | 推奨 | ||||
| 組織 | 推奨 | ||||
| 著者 | 必須 | ||||
| 題名 | 必須 |
(英) Nifedipine induces gingival overgrowth in rats through a reduction in collagen phagocytosis by gingival fibroblasts |
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| 副題 | 任意 | ||||
| 要約 | 任意 |
(英) Nifedipine is used as a long-acting vasodilator, and a primary side effect is the induction of gingival overgrowth, which is characterized by an accumulation of collagenous components within the gingival connective tissue. To elucidate the mechanisms of nifedipine-induced gingival overgrowth, we investigated the effect of nifedipine on Type I collagen metabolism in the gingiva of rats. Twenty-day-old rats were fed a powdered diet containing or lacking nifedipine for 3 to 55 days. Immunohistochemical analysis with anti-Type I collagen antibody was employed to examine the density of Type I collagen in the gingival connective tissue. Total RNAs were isolated from mandibular molar gingiva on days 0, 3, 15, 30, and 55, and reverse transcription polymerase chain reaction was performed to investigate the mRNA levels of Type I collagen. In addition, we performed a flow cytometric analysis with collagen-coated latex beads and cultured fibroblasts derived from rat gingiva to measure collagen phagocytosis. Immunohistochemical analysis revealed that Type I collagen was more prevalent in the connective tissue of nifedipine-treated gingiva than in controls on day 55. In the nifedipine-treated group, the expression of Type I collagen mRNA gradually decreased to 1.5% on day 55 compared to day 0. In the control group, Type I collagen mRNA also decreased to 32%; however, mRNA expression was significantly lower in the nifedipine-treated group than in the controls. When the rate of phagocytic cells derived from nifedipine-treated gingiva and controls was represented as the mean +/- SE of the percentage from 3 different experiments, the values were as follows: on day 15, 13.5 +/- 2.1% and 15.0 +/- 1.5%; on day 30, 12.2 +/- 4.3% and 34.5 +/- 6.7% in the nifedipine-treated and the control group, respectively, indicating that phagocytic cells were considerably fewer in the nifedipine-treated gingiva on day 30. This finding demonstrates that the decrease in phagocytosis caused by nifedipine appeared before the detection of severe macroscopic gingival overgrowth. These findings suggest that the decrease in collagen degradation due to lower phagocytosis is closely associated with the increase in Type I collagen accumulation in nifedipine-treated rat gingiva. |
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| キーワード | 推奨 |
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| 発行所 | 推奨 | (英) American Academy of Periodontology | |||
| 誌名 | 必須 |
Journal of Periodontology(American Academy of Periodontology)
(pISSN: 0022-3492, eISSN: 1943-3670)
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| 巻 | 必須 | 72 | |||
| 号 | 必須 | 8 | |||
| 頁 | 必須 | 1078 1083 | |||
| 都市 | 任意 | ||||
| 年月日 | 必須 | 2001年 8月 初日 | |||
| URL | 任意 | http://www.joponline.org/doi/abs/10.1902/jop.2001.72.8.1078 | |||
| DOI | 任意 | 10.1902/jop.2001.72.8.1078 (→Scopusで検索) | |||
| PMID | 任意 | 11525441 (→Scopusで検索) | |||
| CRID | 任意 | ||||
| WOS | 任意 | ||||
| Scopus | 任意 | ||||
| 評価値 | 任意 | ||||
| 被引用数 | 任意 | ||||
| 指導教員 | 推奨 | ||||
| 備考 | 任意 |
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