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著作: [姚 陳娟]/Karabasil, Ratko, Mileva/Purwanti, Nunuk/[李 雪飛]/[赤松 徹也]/[金森 憲雄]/[細井 和雄]/Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice/[The Journal of Biological Chemistry]

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EID
132634
EOID
667219
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LastModified
2012年10月24日(水) 16:05:05
Operator
大家 隆弘
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細井 和雄
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)
著者 必須
  1. 姚 陳娟([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔分子生理学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Karabasil, Ratko, Mileva / (読) カラバシル ラトコ ミレーバ
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Purwanti, Nunuk / (読) プルワンティ ヌヌク
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. 李 雪飛
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. 赤松 徹也([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.生物資源産業学部.生物資源産業学科.応用生命講座])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 金森 憲雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 細井 和雄
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Tissue kallikrein mK13 is a candidate processing enzyme for the precursor of interleukin-1b in the submandibular gland of mice

副題 任意
要約 任意

(英) By using Western blot analysis, high levels of 17.5- and 20-kDa interleukin-1beta (IL-1beta) proteins were detected in the submandibular gland (SMG) of mice. Despite this fact, the amount of pro-IL-1beta protein, a precursor of IL-1beta, with a molecular size of 35 kDa in this tissue was below the detectable level, although strong expression of pro-IL-1beta mRNA was observed. A large amount of 17.5-kDa IL-1beta also appeared in the saliva of mice injected with lipopolysaccharide, suggesting that this IL-1beta is a secretory form produced by the SMG. The protein for IL-1beta-converting enzyme, a processing enzyme for pro-IL-1beta, was expressed only at a low level in the SMG as compared with its level in various epithelial tissues or lipopolysaccharide-stimulated macrophages. On the other hand, mK1, mK9, mK13, and mK22, members of the kallikrein family, were detected strongly in the SMG but not in other tissues. By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors. A peptide segment corresponding to amino acid residues 107-121 of mouse pro-IL-1beta (107WDDDDNLLVCDVPIR) was cleaved by incubation with mK13, generating two peptides, 107WDDDDNL and 114LVCDVPIR. Therefore, kallikrein mK13 would appear to hydrolyze pro-IL-1beta between its Leu113 and Leu114 residues. The results of immunohistochemistry and an autonomic therapy experiment showed that IL-1beta and kallikrein mK13 were co-localized in the secretory granules of granular convoluted tubular cells. Our present results thus suggest kallikrein mK13 is a plausible candidate for the processing enzyme for pro-IL-1beta in the SMG of mice.

キーワード 推奨
  1. (英) Animals
  2. (英) Blotting, Western
  3. (英) Caspases
  4. (英) Electrophoresis
  5. (英) Enzyme Inhibitors
  6. (英) Enzyme-Linked Immunosorbent Assay
  7. (英) Epithelium
  8. (英) Hydrolysis
  9. (英) Immunoblotting
  10. (英) Immunohistochemistry
  11. (英) Interleukin-1
  12. (英) Isoenzymes
  13. (英) Kallikreins
  14. (英) Leucine
  15. (英) Lipopolysaccharides
  16. (英) Macrophages
  17. (英) Mice
  18. (英) Mice, Inbred C3H
  19. (英) Models, Genetic
  20. (英) Peptides
  21. (英) Phenylmethylsulfonyl Fluoride
  22. (英) Protease Inhibitors
  23. (英) Protein Binding
  24. (英) RNA, Messenger
  25. (英) Reverse Transcriptase Polymerase Chain Reaction
  26. (英) Saliva
  27. (英) Serine Endopeptidases
  28. (英) Submandibular Gland
  29. (英) Time Factors
  30. (英) Tissue Kallikreins
発行所 推奨 The American Society for Biochemistry and Molecular Biology
誌名 必須 The Journal of Biological Chemistry([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
ISSN 任意 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 281
必須 12
必須 7968 7976
都市 任意
年月日 必須 2006年 3月 初日
URL 任意
DOI 任意 10.1074/jbc.M507705200    (→Scopusで検索)
PMID 任意 16423834    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Department of Molecular Oral Physiology, Institute of Health Biosciences, the University of Tokushima Graduate School, 3-18-15, Kuramoto-Cho, Tokushima-Shi, Tokushima 770-8504, Japan.

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't