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著作: Tsuge Hideaki/[川上 竜巳]/[櫻庭 春彦]/Ago Hideo/Miyano Masashi/Aki Kenji/Katunuma Nobuhiko/[大島 敏久]/Crystal Structure of a Novel FAD-, FMN-, and ATP-containing L-Proline Dehydrogenase Complex from Pyrococcus horikoshii/[The Journal of Biological Chemistry]

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EID
123544
EOID
669128
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0
LastModified
2012年11月3日(土) 18:00:05
Operator
大家 隆弘
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TRUE
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[学科長]/[徳島大学.工学部.生物工学科]
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
  1. 徳島文理大学
  2. 徳島大学.工学部.生物工学科.生物反応工学講座
著者 必須
  1. (英) Tsuge Hideaki
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 川上 竜巳([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系.食料科学分野]/[徳島大学.生物資源産業学部])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. 櫻庭 春彦
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Ago Hideo
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Miyano Masashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. (英) Aki Kenji
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. (英) Katunuma Nobuhiko
    役割 任意
    貢献度 任意
    学籍番号 推奨
  8. 大島 敏久([九州大学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Crystal Structure of a Novel FAD-, FMN-, and ATP-containing L-Proline Dehydrogenase Complex from Pyrococcus horikoshii

副題 任意
要約 任意

(英) Two novel types of dye-linked L-proline dehydrogenase complex (PDH1 and PDH2) were found in a hyperthermophilic archaeon, Pyrococcus horikoshii OT3. Here we report the first crystal structure of PDH1, which is a heterooctameric complex (alphabeta)4 containing three different cofactors: FAD, FMN, and ATP. The structure was determined by x-ray crystallography to a resolution of 2.86 angstroms. The structure of the beta subunit, which is an L-proline dehydrogenase catalytic component containing FAD as a cofactor, was similar to that of monomeric sarcosine oxidase. On the other hand, the alpha subunit possessed a unique structure composed of a classical dinucleotide fold domain with ATP, a central domain, an N-terminal domain, and a Cys-clustered domain. Serving as a third cofactor, FMN was located at the interface between the alpha and beta subunits in a novel configuration. The observed structure suggests that FAD and FMN are incorporated into an electron transfer system, with electrons passing from the former to the latter. The function of ATP is unknown, but it may play a regulatory role. Although the structure of the alpha subunit differs from that of the beta subunit, except for the presence of an analogous dinucleotide domain with a different cofactor, the structural characteristics of PDH1 suggest that each represents a divergent enzyme that arose from a common ancestral flavoenzyme and that they eventually formed a complex to gain a new function. The structural characteristics described here reveal the PDH1 complex to be a unique diflavin dehydrogenase containing a novel electron transfer system.

キーワード 推奨
  1. (英) Adenosine Triphosphate
  2. (英) Archaeal Proteins
  3. (英) Crystallography, X-Ray
  4. (英) Flavin Mononucleotide
  5. (英) Flavin-Adenine Dinucleotide
  6. (英) Models, Molecular
  7. (英) Multienzyme Complexes
  8. (英) Proline Oxidase
  9. (英) Protein Structure, Quaternary
  10. (英) Protein Subunits
  11. (英) Pyrococcus horikoshii
発行所 推奨 The American Society for Biochemistry and Molecular Biology
誌名 必須 The Journal of Biological Chemistry([The American Society for Biochemistry and Molecular Biology])
(pISSN: 0021-9258, eISSN: 1083-351X)
ISSN 任意 0021-9258
ISSN: 0021-9258 (pISSN: 0021-9258, eISSN: 1083-351X)
Title: The Journal of biological chemistry
Title(ISO): J Biol Chem
Publisher: American Society for Biochemistry and Molecular Biology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 280
必須 35
必須 31045 31049
都市 任意
年月日 必須 2005年 9月 初日
URL 任意
DOI 任意 10.1074/jbc.C500234200    (→Scopusで検索)
PMID 任意 16027125    (→Scopusで検索)
NAID 任意
WOS 任意
Scopus 任意
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被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.Affiliation: Institute for Health Sciences, Tokushima Bunri University, 180 Nishihama-bouji, Yamashiro-cho, Tokushima 770-8514, Japan. tsuge@tokushima.bunri-u.ac.jp

  2. (英) Article.DataBankList.DataBank.DataBankName: PDB

  3. (英) Article.DataBankList.DataBank.AccessionNumberList.AccessionNumber: 1Y56

  4. (英) Article.PublicationTypeList.PublicationType: Journal Article

  5. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't