徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.大学院ヘルスバイオサイエンス研究部.生体システム栄養科学部門.摂食機能制御学講座(2004年4月1日〜2015年3月31日)

1. 姚 陳娟([徳島大学.大学院医歯薬学研究部.歯学域.口腔科学部門.基礎歯学系.口腔生理学])

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2. 李 雪飛

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3. (英) Kwartarini Murdiastuti

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4. 小杉 知里

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5. 赤松 徹也([徳島大学.大学院社会産業理工学研究部.生物資源産業学域.応用生命系.生体分子機能学分野]/[徳島大学.大学院社会産業理工学研究部.生物資源産業学域.食料科学系])

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6. 金森 憲雄

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7. 細井 和雄

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(英) Lipopolysaccharide-induced elevation and secretion of Interleukin-1b in the submandibular gland of male mice

(英) The intraperitoneal injection of lipopolysaccharide (LPS) (400 microg/kg body weight) induced the expression of mRNAs of inflammatory cytokines such as interleukin (IL)-1beta, IL-6 and tumour necrosis factor (TNF)-alpha in the submandibular gland (SMG) of C3H/HeN mice but not that of C3H/HeJ mice, a mutant strain for Toll-like receptor-4 (TLR-4(-) mutant). The mRNA levels of these cytokines in the SMG of the wild-type mice increased as early as 3 hr after injection, peaked at 3-6 hr, and had decreased again by 24 hr. In this study, we particularly focused on IL-1beta, and induction by this endotoxin was investigated in detail. Denervation of the superior cervical trunk and chorda tympani nerve did not diminish the LPS-induced elevation of IL-1beta mRNA in the SMG, indicating the irrelevance of the central nervous system in this induction. TLR-4 mRNA and protein were shown to be strongly expressed in the SMG, suggesting the direct action of LPS on this gland. IL-1beta proteins were localized in the secretory granules of granular convoluted tubular (GCT) cells, and their molecular weights in the gland were 17.5 and 20 kDa. IL-1beta of the same size appeared in the saliva 6 hr after LPS injection in C3H/HeN but not in C3H/HeJ mice. The present study thus suggests that IL-1beta, an inflammation cytokine, is induced and secreted into the saliva in response to endotoxin injected intraperitoneally.

1. (英) Animals
2. (英) Blotting, Western
3. (英) Cytokines
4. (英) Gene Expression Regulation
5. (英) Injections, Intraperitoneal
6. (英) Interleukin-1
7. (英) Lipopolysaccharides
8. (英) Male
9. (英) Mice
10. (英) Mice, Inbred C3H
11. (英) RNA, Messenger
12. (英) Reverse Transcriptase Polymerase Chain Reaction
13. (英) Saliva
14. (英) Submandibular Gland

1. (英) Article.Affiliation: Department of Molecular Oral Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima-shi, Tokushima, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) OtherID: PMC1817826