徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.医学部.附属動物実験施設(->組織[徳島大学.大学院ヘルスバイオサイエンス研究部.総合研究支援センター.動物資源研究部門])

1. (英) Sakai T

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2. (英) Agui T

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3. 松本 耕三

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(英) Expression of major histocompatibility complex class II but not of CD8 molecules by lectin-stimulated peripheral CD4+ T cells in LEC mutant rats.

(英) LEC rats show a congenital maturational arrest from CD4+8+ to CD4+8- but not to CD4-8+ cells in the thymus. However, some CD4+ cells exist in peripheral lymph nodes. In normal F344 rats, a part of CD4+ T cells expressed CD8 molecules upon concanavaline A (Con A) stimulation. The percentage of CD4+8+ cells and the level of CD8 expression in F344 rat CD4+ cells were enhanced by the glucocorticoid hormone dexamethasone. In contrast, although LEC rat CD4+ cells induced DNA synthesis normally upon Con A stimulation, expression of CD8 was significantly reduced. Furthermore, addition of dexamethasone did not restore the inhibition of CD8 expression. However, LEC rat CD4+ cells could induce CD8 expression, when stimulated by T cell receptor (TCR) cross-linking with anti-TCR monoclonal antibody, suggesting that the TCR-mediated signal is normally transduced in LEC rat CD4+ cells. On the other hand, LEC rat CD4+ cells showed normal expression of major histocompatibility complex (MHC) class II antigens, when stimulated by either Con A or TCR cross-linking, indicating that signals for the induction of MHC class II expression are normal in LEC rat CD4+ cells. Activation of peripheral CD4+ cells has been reported to induce simultaneous expression of CD8 and MHC class II molecules in rats. However, our results using LEC rat CD4+ cells suggest that Con A-induced signaling pathways for CD8 and MHC class II expression can be separable.

1. (英) Animals
2. (英) Antigens, CD8
3. (英) CD4-Positive T-Lymphocytes
4. (英) Cells, Cultured
5. (英) Concanavalin A
6. (英) DNA Replication
7. (英) Dexamethasone
8. (英) Flow Cytometry
9. (英) Histocompatibility Antigens Class II
10. (英) Rats
11. (英) Rats, Inbred F344
12. (英) Rats, Mutant Strains
13. (英) Receptors, Antigen, T-Cell

1. (英) Article.Affiliation: Institute for Animal Experimentation, University of Tokushima School of Medicine, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't