徳島大学 教育・研究者情報データベース(EDB)

Education and Research Database (EDB), Tokushima University

徳島大学ウェブサイトへのリンク

著作: [香美 祥二]/[近藤 秀治]/Loster Klemens/Reutter Werner/Kuhara Takashi/[安友 康二]/[黒田 𣳾弘]/Alpha1beta1 integrin-mediated collagen matrix remodeling by rat mesangial cells is differentially regulated by transforming growth factor-beta and platelet-derived growth factor-BB/[Journal of the American Society of Nephrology]

ヘルプを読む

「著作」(著作(著書,論文,レター,国際会議など))は,研究業績にかかる著作(著書,論文,レター,国際会議など)を登録するテーブルです. (この情報が属するテーブルの詳細な定義を見る)

  • 項目名の部分にマウスカーソルを置いて少し待つと,項目の簡単な説明がツールチップ表示されます.

この情報をEDB閲覧画面で開く

EID
101472
EOID
848388
Map
0
LastModified
2017年2月24日(金) 13:39:59
Operator
三木 ちひろ
Avail
TRUE
Censor
0
Owner
安友 康二
Read
継承
Write
継承
Delete
継承
種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 香美 祥二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.内科系.小児科学]/[徳島大学.病院.診療科.小児·周産·女性科])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. 近藤 秀治([徳島大学.病院.中央診療施設等.地域小児科診療部])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Loster Klemens
    役割 任意
    貢献度 任意
    学籍番号 推奨
  4. (英) Reutter Werner
    役割 任意
    貢献度 任意
    学籍番号 推奨
  5. (英) Kuhara Takashi
    役割 任意
    貢献度 任意
    学籍番号 推奨
  6. 安友 康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  7. 黒田 𣳾弘
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) Alpha1beta1 integrin-mediated collagen matrix remodeling by rat mesangial cells is differentially regulated by transforming growth factor-beta and platelet-derived growth factor-BB

副題 任意
要約 任意

(英) Pathologic remodeling of mesangial matrix after glomerular injury is the central biologic feature of glomerular scarring (sclerosis). Transforming growth factor-beta (TGF-beta) and platelet-derived growth factor (PDGF)-BB have been implicated in the development of glomerular scarring in rat and human glomerulonephritis. To clarify molecular and cellular mechanisms involved in abnormal mesangial remodeling, this study focused on the role of alpha1beta1 integrin, a collagen/laminin receptor, in rat mesangial cells, using collagen gel contraction as an experimental model of in vivo collagen matrix remodeling and scar formation. In addition, the influence of TGF-beta and PDGF-BB on mesangial cell (MC)-mediated collagen gel contraction in association with the alpha1beta1 integrin expression was evaluated. Integrin function blocking studies using anti-alpha1, beta1 subunit antibodies indicated that MC-alpha1beta1 integrin is essentially required not only for collagen-dependent adhesion/migration, but also for gel contraction. Protein synthesis and mRNA analysis experiments demonstrated that TGF-beta, but not PDGF-BB, increases the expression of alpha1beta1 integrin in mesangial cells cultured on plastic surface and in collagen gels. The upregulation of alpha1beta1 integrin expression by TGF-beta correlated with increases in gel contraction and collagen-dependent adhesion but not migration of mesangial cells. On the other hand, PDGF-BB enhanced MC-mediated gel contraction and migration without affecting cell adhesion to collagen I. Growth factor-induced collagen-dependent adhesion, migration, and gel contraction were significantly attenuated by incubation with anti-alpha1, beta1 subunit antibodies. Thus, these data indicate that alpha1beta1 integrin-mediated collagen matrix remodeling can be modulated by TGF-beta and PDGF-BB via different mechanisms. Alpha1 integrin-mediated mesangial matrix remodeling induced by TGF-beta or PDGF-BB may be a pathogenic mechanism leading to glomerular scarring.

キーワード 推奨
  1. (英) Animals
  2. (英) Blotting, Northern
  3. (英) Cell Adhesion
  4. (英) Cell Movement
  5. (英) Cells, Cultured
  6. コラーゲン(collagen)
  7. (英) Disease Models, Animal
  8. 細胞外マトリックス(extracellular matrix)
  9. (英) Glomerular Mesangium
  10. (英) Humans
  11. 免疫組織化学(immunohistochemistry)
  12. (英) Integrin alpha1beta1
  13. (英) Integrins
  14. (英) Platelet-Derived Growth Factor
  15. (英) Rats
  16. (英) Rats, Sprague-Dawley
  17. (英) Reference Values
  18. (英) Transforming Growth Factor beta
発行所 推奨 (英) American Society of Nephrology
誌名 必須 Journal of the American Society of Nephrology(American Society of Nephrology)
(pISSN: 1046-6673, eISSN: 1533-3450)
ISSN 任意 1046-6673
ISSN: 1046-6673 (pISSN: 1046-6673, eISSN: 1533-3450)
Title: Journal of the American Society of Nephrology : JASN
Title(ISO): J. Am. Soc. Nephrol.
Publisher: American Society of Nephrology
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 10
必須 4
必須 779 789
都市 任意
年月日 必須 1999年 4月 初日
URL 任意 http://jasn.asnjournals.org/cgi/content/abstract/10/4/779
DOI 任意
PMID 任意 10203362    (→Scopusで検索)
NAID 任意
WOS 任意 000079365900012
Scopus 任意 2-s2.0-0032588651
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Comparative Study

  2. (英) Article.PublicationTypeList.PublicationType: Journal Article

  3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't