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著作: [安友 康二]/Lucas Bruno/Germain N Ronald/TCR signaling for initiation and completion of thymocyte positive selection has distinct requirements for ligand quality and presenting cell type/[The Journal of Immunology]

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EID
101464
EOID
732250
Map
0
LastModified
2014年4月30日(水) 14:10:15
Operator
松井 栄里
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TRUE
Censor
0
Owner
安友 康二
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種別 必須 学術論文(審査論文)
言語 必須 英語
招待 推奨
審査 推奨 Peer Review
カテゴリ 推奨
共著種別 推奨
学究種別 推奨
組織 推奨
著者 必須
  1. 安友 康二([徳島大学.大学院医歯薬学研究部.医学域.医科学部門.病理系.生体防御医学])
    役割 任意
    貢献度 任意
    学籍番号 推奨
  2. (英) Lucas Bruno
    役割 任意
    貢献度 任意
    学籍番号 推奨
  3. (英) Germain N Ronald
    役割 任意
    貢献度 任意
    学籍番号 推奨
題名 必須

(英) TCR signaling for initiation and completion of thymocyte positive selection has distinct requirements for ligand quality and presenting cell type

副題 任意
要約 任意

(英) Thymocyte selection involves signaling by TCR engaging diverse self-peptide:MHC molecule ligands on various cell types in the cortex and medulla. Here we separately analyze early and late stages of selection to better understand how presenting cell type, ligand quality, and the timing of TCR signaling contribute to intrathymic differentiation. TCR transgenic CD4+CD8+ thymocytes (double positive (DP)) from MHC-deficient mice were stimulated using various presenting cells and ligands. The resulting CD69high cells were isolated and evaluated for maturation in reaggregate cultures with wild-type or MHC molecule-deficient thymic stroma with or without added hemopoietic dendritic cells (DC). Production of CD4+ T cells required TCR signaling in the reaggregates, indicating that transient recognition of self-ligands by DP is inadequate for full differentiation. DC bearing a potent agonist ligand could initiate positive selection, producing activated thymocytes that matured into agonist-responsive T cells in reaggregates lacking the same ligand. DC could also support the TCR signaling necessary for late maturation. These results argue that despite the negative role assigned to DC in past studies, neither the peptide:MHC molecule complexes present on DC nor any other signals provided by these cells stimulate only thymocyte death. These findings also indicate that unique epithelial ligands are not necessary for positive selection. They provide additional insight into the role of ligand quality in selection events and support the concept that following initiation of maturation from the DP state, persistent TCR signaling is characteristic of and perhaps required by T cells.

キーワード 推奨
  1. (英) Animals
  2. (英) Antigen-Presenting Cells
  3. (英) Antigens, CD
  4. (英) Antigens, CD4
  5. (英) Antigens, CD8
  6. (英) Antigens, Differentiation, T-Lymphocyte
  7. (英) CD4-Positive T-Lymphocytes
  8. (英) Cell Aggregation
  9. (英) Cell Differentiation
  10. (英) Cell Survival
  11. (英) Cells, Cultured
  12. (英) Cytochrome c Group
  13. (英) Dendritic Cells
  14. (英) Histocompatibility Antigens Class II
  15. (英) Immunophenotyping
  16. (英) Lectins, C-Type
  17. (英) Ligands
  18. (英) Lymphocyte Activation
  19. (英) Major Histocompatibility Complex
  20. (英) Mice
  21. (英) Mice, Inbred C57BL
  22. (英) Mice, Knockout
  23. (英) Mice, Transgenic
  24. (英) Peptides
  25. (英) Proto-Oncogene Proteins c-bcl-2
  26. (英) Receptors, Antigen, T-Cell
  27. (英) Signal Transduction
  28. (英) Stromal Cells
  29. (英) T-Lymphocyte Subsets
  30. (英) Thymus Gland
  31. (英) Up-Regulation
発行所 推奨
誌名 必須 The Journal of Immunology([The American Association of Immunologists])
(pISSN: 0022-1767, eISSN: 1550-6606)
ISSN 任意 0022-1767
ISSN: 0022-1767 (pISSN: 0022-1767, eISSN: 1550-6606)
Title: Journal of immunology (Baltimore, Md. : 1950)
Title(ISO): J Immunol
Publisher: American Association of Immunologists
 (NLM Catalog  (Scopus  (CrossRef (Scopus information is found. [need login])
必須 165
必須 6
必須 3015 3022
都市 任意
年月日 必須 2000年 9月 15日
URL 任意 http://jimmunol.org/cgi/content/abstract/165/6/3015
DOI 任意
PMID 任意 10975810    (→Scopusで検索)
NAID 任意
WOS 任意 000165938100014
Scopus 任意 2-s2.0-0034665493
評価値 任意
被引用数 任意
指導教員 推奨
備考 任意
  1. (英) Article.PublicationTypeList.PublicationType: Journal Article

  2. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't