徳島大学 教育・研究者情報データベース(EDB)

1. 徳島大学.医学部.附属動物実験施設(->組織[徳島大学.大学院ヘルスバイオサイエンス研究部.総合研究支援センター.動物資源研究部門])

1. (英) Okamura Kenji

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2. (英) Nakamura Kimitoshi

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3. (英) Hisatomi Yuichiro

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4. (英) Nagano Koji

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5. (英) Tanaka Yasuhiko

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6. (英) Terada Kunihiko

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7. (英) Sugiyama Toshihiro

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8. (英) Umeyama Kazuhiro

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9. 松本 耕三

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10. (英) Yamamoto Tetsuro

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11. (英) Endo Fumio

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(英) Salivarygland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineage.

(英) Tissue damage can be assessed based on regenerative responses, including progenitor cell proliferation. In the salivary gland, tissue damage induced by ligation of main ducts leads to the disappearance of acinar cells and to marked proliferation of ductal cells. Reopening of the ducts leads to repopulation of acinar cells within 1 to 2 weeks, which suggests activation of tissue progenitor cells in a damaged state. Because submandibular glands derive from the endoderm and ectoderm, we investigated the possibility of the presence of endodermal progenitor cells. We cultured cells obtained from the ligated salivary gland and identified colonies of epithelium-like cells. We singled out and purified the cells by limited dilution, and one of the cells designated SGP-1 was used for further experiments. The SGP-1 expresses both alpha6beta1 integrin and cytoplasmic laminin. The hematopoietic stem cell marker CD34 and hepatic oval cell markers such as albumin, alpha-fetoprotein (AFP), and cytokeratin 19 are all negative. However, when SGP-1 cells were transplanted into the liver via the portal vein, these cells were integrated into hepatic trabecula and produced albumin. When SGP-1 cells formed clusters on type I collagen-coated dishes, they differentiated into endodermal lineage and 2 major types of clusters appeared: one contained cells positive for AFP and/or albumin (hepatic cluster) and the other positive for glucagon and/or insulin (pancreatic cluster). On laminin-coated dishes, SGP-1 selectively differentiated into hepatic-type cells. In conclusion, the multipotent progenitor cells isolated from the rat salivary gland have characteristics of tissue stem cells and can differentiate into cells of endodermal lineages.

1. (英) Animals
2. (英) Cell Differentiation
3. (英) Cell Lineage
4. (英) Cell Separation
5. (英) Cells, Cultured
6. (英) Endoderm
7. (英) Hepatocytes
8. (英) Ligation
9. (英) Pancreas
10. (英) Rats
11. (英) Rats, Inbred LEC
12. (英) Rats, Sprague-Dawley
13. (英) Salivary Ducts
14. (英) Stem Cell Transplantation
15. (英) Stem Cells
16. (英) Submandibular Gland

1. (英) Article.Affiliation: Department of Pediatrics, School of Medicine, Kumamoto University, Kumamoto, Japan.

2. (英) Article.PublicationTypeList.PublicationType: Journal Article

3. (英) Article.PublicationTypeList.PublicationType: Research Support, Non-U.S. Gov't